منابع مشابه
Preparation of pure lipid hydroperoxides.
Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...
متن کاملPreparation of pure oligonucleotide-alkaline phosphatase conjugates.
The utility and sensitivity of enzyme-labeled oligonucleotide probes have been described (1, 2). However, most methods used for their preparation require the use of extensive column chromatography, polyacrylamide gel electrophoresis, or high-pressure liquid chromatography to yield a pure product (3-5). Commercial kits offer a simple alternative but typically 1) are expensive, 2) utilize freeze ...
متن کاملThe Preparation of Relatively Pure Bacteriophage
The method described above, based on the electrophoretic migration of bacteriophage particles into an agar gel and their subsequent re-suspension in a suitable medium, has the following advantages: It is simple and can be readily carried out on a comparatively large scale by merely inserting additional units between the same electrode cups. It requires but one extraction and the resulting phage...
متن کاملOn the Preparation and Properties of Pure Glutathione (glutaminyl-cysteine)
Glutathione was first. isolated in 1921 from yeast, mammalian liver, and muscle by Hopkins (5), who also presented evidence indicating that the substance is a dipeptide of cysteine and glutamic acid. In 1925 Stewart and Tunnicliffe (8) published their work on the synthesis of glutathione. Their second synthetic method yielded a product, which possessed all of the properties of the substance fou...
متن کاملA pure preparation of the staphylococcal typing bacteriophage-42-D.
The staphylococcal typing-phage 42 D is propagated on bacteria which are lysogenic for the phage 1363, and the latter phage is present as a contaminant in preparations of phage 42D. This contamination can be avoided by propagating a pure clone of phage 42D on non-lysogenic organisms. Such nonlysogenic propagating strains can be isolated after the lysogenic propagating organisms have been irradi...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1963
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.17-2347